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Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

DOI :

10.3791/53392-v

April 29th, 2016

April 29th, 2016

8,330 Views

1Temasek Life Sciences Laboratory, 2Department of Biological Science, National University of Singapore, 3Bioimaging and Biocomputing Facility, Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core, Gladstone Institutes, 5Pacific Biosciences Research Center, University of Hawai'i at Mānoa

The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.

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