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Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells

DOI :

10.3791/54176-v

July 22nd, 2016

July 22nd, 2016

18,225 Views

1Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miller School of Medicine, 2Department of Cell Biology, University of Miami, Miller School of Medicine

Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.

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