March 26th, 2010
•Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
Tags
Related Videos
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Induction and Analysis of Epithelial to Mesenchymal Transition
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved