Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
10.5K Views
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12:21 min
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July 25th, 2016
DOI :
July 25th, 2016
•Hananeh Fonoudi*1,2,3,8, Hassan Ansari*1,8, Saeed Abbasalizadeh1, Gillian M Blue6,7, Nasser Aghdami1, David S Winlaw6,7, Richard P Harvey2,3,4, Alexis Bosman*2,3, Hossein Baharvand*1,5
1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, 4School of Biotechnology and Biomolecular Sciences, University of New South Wales, 5Department of Developmental Biology, University of Science and Culture, 6Heart Centre for Children, The Children´s Hospital at Westmead, 7Sydney Medical School, University of Sydney, 8Department of Developmental Biology, University of Science and Culture, Tehran, Iran
Chapters in this video
0:05
Title
1:03
Spinner Flask Internal Surface Silicon Coating
2:42
hPSC Spheroid Stirred Suspension Bioreactor Differentiation
6:42
hPSC Differentiation Using Cultures Not Adapted to Single Cell Passaging
9:46
Results: Representative Cardiomyocyte Differentiation From Various hPSCs Cultures
11:19
Conclusion
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