Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
4.0K Views
•
05:38 min
•
February 12th, 2021
DOI :
February 12th, 2021
•Shiqiao Ye1,2, Xiaoping Wan3, Juan Su1,2, Akshar Patel1,2,4, Blake Justis1,2,5, Isabelle Deschênes3, Ming-Tao Zhao1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine
Chapters in this video
0:04
Introduction
1:08
Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging
1:55
Chemically Defined Cardiomyocyte (CM) Differentiation
2:57
Results: Representative iPSC-CM Differentiation and Function
4:51
Conclusion
Related Videos
08:00
Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
8.1K Views
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
7.3K Views
10:16
Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
7.7K Views
10:12
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
16.9K Views
09:05
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
10.9K Views
08:37
Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells
6.9K Views
10:46
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
19.8K Views
10:37
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
6.3K Views
09:23
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
3.6K Views
13:18
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
21.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved