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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

DOI :

10.3791/54278-v

10:52 min

August 13th, 2016

August 13th, 2016

9,297 Views

1Department of Bionanoscience, Delft University of Technology

The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.

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Reconstitution

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