August 13th, 2016
•The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
Related Videos
Title Cell Encapsulation by Droplets
Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
In vitro Reconstitution of the Active T. castaneum Telomerase
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
Basic Caenorhabditis elegans Methods: Synchronization and Observation
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved