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Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers

DOI :

10.3791/63968-v

11:55 min

July 12th, 2022

July 12th, 2022

1,479 Views

1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research

This protocol describes the formation of supported lipid bilayers and the addition of cytoskeletal filaments and motor proteins to study the dynamics of reconstituted, membrane-tethered cytoskeletal networks using fluorescence microscopy.

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Membrane tethered Actin Cortices

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