JoVE Logo
Faculty Resource Center

Sign In

RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

DOI :

10.3791/54280-v

September 27th, 2016

September 27th, 2016

7,335 Views

1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center

Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

Tags

RNA Interference

-- Views

Related Videos

article

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

article

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

article

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

article

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

article

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

article

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

article

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

article

An Assay for Quantifying Protein-RNA Binding in Bacteria

article

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

article

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved