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RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

DOI :

10.3791/54280-v

September 27th, 2016

September 27th, 2016

7,279 Views

1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center

Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

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