This paper described a simple method for assaying wounding in a three-dimensional, multi-cellular organ culture model system. We do this by using pig eyes, but even if you are not familiar with eyes, you can do this assay. As an overview, we recei
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A protocol for an ex vivo corneal organ culture model useful for wound healing studies is described. This model system can be used to assess the effects of agents to promote regenerative healing or drug toxicity in an organized 3D multicellular environment.