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Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

DOI :

10.3791/61471-v

7:00 min

June 24th, 2020

June 24th, 2020

20,586 Views

1IRIBHM, Université Libre de Bruxelles (ULB), 2Medecine Faculty, Université Libre de Bruxelles (ULB)

Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.

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Nuclei Isolation

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