JoVE Logo
Faculty Resource Center

Sign In

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

DOI :

10.3791/55528-v

July 28th, 2017

July 28th, 2017

12,601 Views

1Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science, University of Minnesota, 3Department of Plant Pathology, University of Minnesota

The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

-- Views

Related Videos

article

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

article

Exercise Test for Evaluation of the Functional Efficacy of the Pig Cardiovascular System

article

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

article

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

article

Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains

article

Bleaching and Plating Caenorhabditis elegans P0 Generation Embryos onto RNAi-NGM plates

article

Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression

article

Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved