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Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

DOI :

10.3791/54405-v

August 31st, 2016

August 31st, 2016

5,462 Views

1Cell and Developmental Biology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

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