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Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

DOI :

10.3791/54406-v

9:25 min

August 19th, 2016

August 19th, 2016

10,506 Views

1Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame

Here, a method using a permeable membrane insert-based infection system to study the effects of Streptolysin S, a secreted toxin produced by Group A Streptococcus, on keratinocytes is described. This system can be readily applied to the study of other secreted bacterial proteins on various host cell types during infection.

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Permeable Membrane Insert based Infection System

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