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Visualizing Bacteria in Nematodes using Fluorescent Microscopy

DOI :

10.3791/4298-v

October 19th, 2012

October 19th, 2012

18,399 Views

1Department of Bacteriology, University of Wisconsin-Madison

To study the mutualism between Xenorhabdus bacteria and Steinernema nematodes, methods were developed to monitor bacterial presence and location within nematodes. The experimental approach, which can be applied to other systems, entails engineering bacteria to express the green fluorescent protein and visualizing, using fluorescence microscopy bacteria within the transparent nematode.

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