Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
11.0K Views
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08:14 min
•
November 13th, 2015
DOI :
November 13th, 2015
•Harald Lahm*1, Stefanie A. Doppler*1, Martina Dreßen1, Klaudia Adamczyk1, Marcus-André Deutsch1, Hanna Ulrich2, Matthias Schiemann2,3, Rüdiger Lange1,4, Markus Krane1,4
1Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, 3Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance
Chapters in this video
0:05
Title
1:41
Cell Culture Treatment with Nanoparticles
3:42
Identification of Cy3-positive Cells via Flow Cytometry
5:53
Results: Detection of Pluripotency Marker Gene Expression in Live iPSCs and Embryonic Stem Cells
7:36
Conclusion
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