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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

DOI :

10.3791/52357-v

8:46 min

January 6th, 2015

January 6th, 2015

31,742 Views

1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source

A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

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Green Fluorescent Protein

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