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Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay

DOI :

10.3791/54443-v

9:49 min

September 6th, 2016

September 6th, 2016

7,461 Views

1Center for Advanced Biotechnology and Medicine, Rutgers University, 2Department of Neuroscience and Cell Biology, Rutgers University

Methods to accurately measure retinoic acid (RA) levels in small amounts of tissue do not exist. This protocol describes the easy, quantitative measurement of relative RA levels in E8.5 embryos and neurospheres using an RA reporter cell line.

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Quantitative Measurement

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