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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

DOI :

10.3791/54473-v

11:19 min

October 9th, 2016

October 9th, 2016

14,297 Views

1Department of Biomolecular Chemistry, University of Wisconsin-Madison, 2Department of Medicine, University of Wisconsin-Madison

Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.

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STAT3 Splice Variant Transcripts

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