Plating the Strains on the Copper Plates, Data Collection, and Analysis
5:09
Results: Impact of Mutant Splicing Factors on the Spliceosomal Function
6:50
Conclusion
Transcript
The ACT1-CUP1 Assay can identify if a mutation affects pre-messenger RNA splicing, and give an indication of which splicing step is most impacted. The ACT1-CUP1 Assay links splicing factor mutation to its impact on the complex process of splicing.
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The ACT1-CUP1 assay, a copper growth assay, provides a quick readout of precursor messenger RNA (pre-mRNA) splicing and the impact mutant splicing factors have on spliceosomal function. This study provides a protocol and highlights the customization possible to address the splicing question of interest.