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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

DOI :

10.3791/54527-v

October 23rd, 2016

October 23rd, 2016

9,989 Views

1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

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