Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
3:18
Effect of HdeB Overexpression on E. coli Survival under Acid Stress
5:14
Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
7:16
Conclusion
Transcript
The overall goal of these in vivo and in vitro chaperone assays is to characterize the pH-dependent activity of the Escherichia coli chaperone HdeB under acidic pH conditions. Acid activated chaperones have only one job to do, they need to protect
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This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.