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Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

DOI :

10.3791/54670-v

November 2nd, 2016

November 2nd, 2016

8,702 Views

1Department of Molecular Biology, Princeton University

Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.

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Drosophila Muscle Tissue Removal

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