JoVE Logo
Faculty Resource Center

Sign In

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

DOI :

10.3791/54703-v

11:22 min

December 1st, 2017

December 1st, 2017

11,868 Views

1Graduate Program in Molecular Biology, Cell Biology, and Biochemistry, Boston University, 2Biological Design Center, Boston University, 3Lattice Automation, 4Department of Electrical and Computer Engineering, Biological Design Center, Boston University

Here, an automated workflow to perform modular DNA "device" assembly using a modular cloning DNA assembly method on liquid-handling robots is presented. The protocol uses an intuitive software tool for generating liquid handler picklists for combinatorial DNA device library generation, which we demonstrate using two liquid handling platforms.

Tags

Automated Robotic Liquid Handling Assembly

-- Views

Related Videos

article

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

article

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

article

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

article

Fabricating Cotton Analytical Devices

article

Preparation of Monodomain Liquid Crystal Elastomers and Liquid Crystal Elastomer Nanocomposites

article

Rod-based Fabrication of Customizable Soft Robotic Pneumatic Gripper Devices for Delicate Tissue Manipulation

article

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

article

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

article

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

article

Handling and Assessment of Human Primary Prostate Organoid Culture

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved