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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

12.3K Views

11:22 min

December 1st, 2017

DOI :

10.3791/54703-v

December 1st, 2017


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Automated DNA Assembly

Chapters in this video

0:05

Title

0:50

Specifying Parts to be Used in DNA Device Library and Generating Using User/Liquid Handler Instructions

2:24

Preparing Plasmid DNA and Reagents for Assembly

3:55

Executing the Assembly Script on the Liquid Handler

5:47

Transforming the Reactions

7:56

Clone Verification

8:50

Results: Automated DNA Assembly Reactions Yield Correct Clones with Comparable Frequency to Reactions Prepared Manually and with Significantly Less Hands-on Time

10:20

Conclusion

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