CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library

776 Views

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05:30 min

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May 17th, 2024

DOI :

10.3791/66581-v

May 17th, 2024

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Si Xu*¹^,², Mengyu Chen*¹^,², Guang Chen¹^,³, Si Luo⁴, Wen Xue¹^,², Xuelian Liu¹^,², Jiwu Wang¹^,²^,³, Ping Wei⁴

¹Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, ²Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, ³Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, ⁴Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

Summary

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CRISPR based Modular Assembly

CRISPRmass

Plasmid Library Construction

Functional Genomics Screening

Genome wide Plasmid Libraries

UAS cDNA ORF

High throughput Construction

Cas9 Technology

Genetic Editing

Bacterial Transformation

GAL4 UAS based Library

SgRNA

Chapters in this video

0:00

Introduction

1:21

Large‐Scale Construction of UAS‐cDNA/ORF Plasmids by CRISPR‐Based Modular Assembly (CRISPRmass)

3:44

Large‐Scale Transformation of UAS‐cDNA/ORF Plasmids from CRISPR‐Based Modular Assembly into E. coli

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