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Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

DOI :

10.3791/54880-v

December 31st, 2016

December 31st, 2016

22,198 Views

1Graduate School of Biomedical Sciences, Rutgers New Jersey Medical School, 2Department of Pharmacology, Physiology and Neuroscience, Rutgers New Jersey Medical School

This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings using biocytin filling and subsequent immunohistochemical postprocessing. We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later.

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Immunostaining

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