Staining of the First Primary Antibody and Biocytin
4:58
Staining of the Second Primary Antibody
6:32
Results: Successful CCK Staining 1 Week Following the Recovery of Biocytin Staining and CB1R Labeling
7:56
Conclusion
Transcript
The goal of this protocol is to present an efficient method for identifying neurons patched during electrophysiological recordings using biocytin and immunostaining for recovery of morphology prior to re-staining with specific antibodies to determ
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This protocol presents a method for the morphological recovery of neurons patched during electrophysiological recordings using biocytin filling and subsequent immunohistochemical postprocessing. We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later.