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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

DOI :

10.3791/54976-v

December 28th, 2016

December 28th, 2016

9,889 Views

1Entomology Department, University of Maryland, 2Program in Molecular and Cell Biology, University of Maryland

Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

Tags

Gene Function

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