May 29th, 2020
•RNA interference is a widely applicable, powerful technique for manipulating gene expression at specific developmental stages. Here, we describe the necessary steps for implementing this technique in the aquatic diving beetle Thermonectus marmoratus, from the acquisition of gene sequences to the knockdown of genes that affect structure or behavior.
Related Videos
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity
Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Alternative Method of Removing Otoliths from Sturgeon
A Flow-through Exposure System for Evaluating Suspended Sediments Effects on Aquatic Life
Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
Characterization of Aquatic Biofilms with Flow Cytometry
The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression
Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved