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Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

DOI :

10.3791/50693-v

September 25th, 2013

September 25th, 2013

11,827 Views

1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

While dsRNA feeding in C. elegans is a powerful tool to assess gene function, current protocols for large scale feeding screens result in variable knockdown efficiencies. We describe an improved protocol for performing large scale RNAi feeding screens that results in highly efficacious and reproducible knockdown of gene expression.

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