Embedding the Condyle and Hind Leg for Frozen Sectioning
5:51
Slide Preparation and Basic Counterstaining
6:29
Immunohistochemical Staining for Runx2 and DMP1
8:04
Results: Cell Lineage Tracing
9:58
Conclusion
Transcript
The overall goal of this cell lineage tracing technique and subsequent immunofluorescence is to investigate cell biology and cell fate in vivo. This method can help answer key questions in the cell biology and tissue development field regarding th
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We developed two sets of tracing combinations in Rosa26tdtomato (ubiquitously expressed in all cells)/Cre (specifically expressed in chondrocytes) mice: one with 2.3Col1a1-GFP (specific to osteoblasts) and one with immunofluorescence (specific to bone cells). The data demonstrate the direct transformation of chondrocytes into bone cells.