JoVE Logo
Faculty Resource Center

Sign In

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

DOI :

10.3791/53790-v

March 20th, 2016

March 20th, 2016

9,486 Views

1The Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, 2The Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey

At the subcellular level, signaling events are dynamically modulated by developmental and environmental cues. Here we describe a protocol that employs the tobacco transient expression system to monitor dynamic protein-protein interaction and to disclose spatial organization of signal transduction in plant cells.

-- Views

Related Videos

article

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

article

Quantifying Mixing using Magnetic Resonance Imaging

article

Spatial Multiobjective Optimization of Agricultural Conservation Practices using a SWAT Model and an Evolutionary Algorithm

article

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

article

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

article

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

article

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

article

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

article

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

article

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved