JoVE Logo
Faculty Resource Center

Sign In

Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation

DOI :

10.3791/55010-v

7:13 min

March 14th, 2017

March 14th, 2017

13,954 Views

1Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, 2West China School of Stomatology, Sichuan University, 3Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, 4State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University

Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor.

Tags

Mouse Models

-- Views

Related Videos

article

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

article

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

article

Robotic Ablation of Atrial Fibrillation

article

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

article

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

article

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

article

Ablation of Ischemic Ventricular Tachycardia Using a Multipolar Catheter and 3-dimensional Mapping System for High-density Electro-anatomical Reconstruction

article

Protocol to Create Chronic Wounds in Diabetic Mice

article

Two-Dimensional X-Ray Angiography to Examine Fine Vascular Structure Using a Silicone Rubber Injection Compound

article

Sleeve Gastrectomy in Mice using Surgical Clips

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved