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Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

DOI :

10.3791/62040-v

7:38 min

April 30th, 2021

April 30th, 2021

2,794 Views

1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca2+ imaging to elucidate Ca2+ transient properties of pacemaker cells and other interstitial cells in vibratome slices.

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Isolating

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