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Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

DOI :

10.3791/55013-v

January 12th, 2017

January 12th, 2017

7,867 Views

1Laboratoire d'écologie et d'épidémioloigie parasitaire, Institut de biologie, Université de Neuchâtel, 2Merkle Lab, Department of Entomology, Pennsylvania State University

Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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Inoculation

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