A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains
27.1K Views
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09:53 min
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December 1st, 2016
DOI :
December 1st, 2016
•1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston, The University of Texas Health Science Center at Houston, 2Department of Natural Sciences, University of Houston - Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School, The University of Texas Health Science Center at Houston
Chapters in this video
0:05
Title
1:12
Microsurgical Dissection of Adult Fly Heads
2:56
Immunofluorescent Staining of the Brains
4:44
Mounting and Imaging of Brain Tissue
6:02
Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
7:40
Conclusion
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