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Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

DOI :

10.3791/55129-v

January 11th, 2017

January 11th, 2017

8,367 Views

1Department of Chemistry, Stony Brook University, 2Department of Physiology and Biophysics, Stony Brook University

We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.

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