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Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

DOI :

10.3791/1669-v

December 23rd, 2009

December 23rd, 2009

16,787 Views

1University of Massachusetts Medical School

Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.

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Plasma Membrane Proteins

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