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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

18.8K Views

25:06 min

September 24th, 2009

DOI :

10.3791/1333-v

September 24th, 2009


Transcript

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In Utero Electroporation

Chapters in this video

0:05

Title

1:07

Introduction

1:46

In utero Retinal Electroporation - Injecting and Electroporating DNA into E14.5 Murine Retinae in vivo

8:40

In utero Retinal Electroporation - Retrieval of Electroporated Retina at E18.5

12:34

Ex vivo Retinal Electroporation - Injecting and Electroporating DNA into E14.5 Murine Retinae in vitro

18:49

Ex vivo Retinal Electroporation - Harvesting and Plating of GFP+ Retinal Explants (2 days post-electroporation)

23:33

Representative Results from in utero and ex vivo Retinal Electroporations

25:23

Conclusion

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