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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

DOI :

10.3791/1333-v

25:06 min

September 24th, 2009

September 24th, 2009

18,427 Views

1Departments of Pathology and Cell Biology, and Neuroscience, Columbia University College of Physicians and Surgeons, 2Department of Ophthalmology, Columbia University College of Physicians and Surgeons

Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.

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In Utero Electroporation

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