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Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

DOI :

10.3791/50271-v

April 3rd, 2013

April 3rd, 2013

12,654 Views

1Department of Neuroscience and Physiology, SUNY Upstate Medical University

This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.

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