cOVA-TH1 Cell Radiolabeling and Uptake and Efflux Analysis
6:44
Positron Emission Tomograph–Computed Tomography (PET/CT) In Vivo Imaging
8:26
Results: Representative cOVA-TH1 Cell Tracking In Vitro and In Vivo
10:32
Conclusion
Transcript
The overall goal of this procedure is to stably label murine lymphocytes with a copper-modified radioactive monoclonal antibody to track lymphocyte temporal distribution and homing patterns in vivo by PET/CT in mouse models of inflammation or canc
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Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).