Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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09:06 min
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May 1st, 2017
DOI :
May 1st, 2017
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Chapters in this video
0:05
Title
1:14
Protein Extraction and Generation of Peptides for Chemical-tagging
2:05
Sample Digestion
3:16
Dimethylation Labeling (N-termini)
5:13
Isobaric Tagging (Lys residues)
6:12
Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) and MS3
7:02
Results: cPILOT Can Be Used to Analyze Tissues from an Alzheimer's Disease Mouse Model and Wild-type Controls
8:23
Conclusion
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