Name: recording eeg in freely moving neonatal rats using a novel method
Uploaded: 2017-05-29T12:30:00
Description: Watch this Scientific Journal Video about Recording EEG in Freely Moving Neonatal Rats Using a Novel Method at JoVE.com

This work was supported by the Natural Science Foundation of China (31171355) and the Natural Science Foundation of Guangdong (S2011010003403, 2014A030313440).

Animal care, surgical procedure, and recording procedures were in accordance with the guidelines for the South China Normal University Animal Care and Use Committee.
1. Electrode Preparation (Figure 1A-C)
NOTE: Computer pin loci is simply a pronged contact as a part of signal interface in communication devices. It consists of a male connector that plugs into the female connector.
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	<li>Carefully separate the male and female pins from the computer pin loci (<st...

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Here, we report surgical and recording procedures to acquire EEG in freely moving neonatal rat pups by wired method (Figure 3). It has been suggested that the P7-P12 rat pup is at the developmental age that corresponds to a full-term human neonate 10,11. It is technically difficult to obtain high-quality EEG recording data when working with rat pups in this age group. In addition, it requires specialized handling and great care ...

It is well established that continuous communication between neurons is required to obtain normal brain function. The interneuronal communication primarily takes place at synapses, where information from one neuron is conveyed to a second neuron. This synaptic transmission is mediated by two types of dedicated structural arrangements: electrical or chemical synapses 1. Electrophysiology is the field that captures the electrical potential produced during interneuronal communication that control overall body functions and behavior 2. EEG is the most commonly used method among many electrophysiological techniques.
EEG is a technique used to detect changes in electrical signals produced by internal or external stimuli. Moreover, it is an essential test for clinical diagnosis and outcome prediction of various neurological conditions such as epilepsy, Parkinson&#39;s and Alzheimer&#39;s disease, as well as effects of pharmacological and toxicological agents 3. Generally, an epileptic patient shows hyperexcitability and impaired functional connectivity within the brain; these are summarized as interictal epileptiform discharges (IEDs) and can be recorded by EEG in the form of sharp, transient spikes; sharp waves; spike-wave complexes; or polyspikes 4. The main feature of the epileptic brain is the spontaneous occurrence of epileptic seizures, which can be recorded either from the scalp or from the brain parenchyma in order to locate the brain area responsible for the seizures 5. Furthermore, EEG also has very important implications in neurodegenerative disorders like Alzheimer&#39;s disease (AD). Research suggests that altered EEG recordings and impaired oscillatory networks in AD patients are common. However, our knowledge about the pathophysiology of network oscillations in neurodegenerative diseases is surprisingly incomplete and needs to be further explored 6.
In this protocol, we have designed a simple electrode with which one can record EEG to understand the electrical communication in both the normal and the pathological brain. The surgical implantation in this method is cheaper than other available procedures 7. Moreover, this method can be used to record high-quality and stable EEG signals for longer timeframes (i.e., 2-4 h every day for 1 week). In addition, we used lighter electrodes (weighing approximately 26 mg) that enable the animals to behave more naturally 8. This method is widely applicable to the study of EEG in neonatal rat pups that requires the amplifier and digitizer, commonly used in electrophysiology lab and does not require any additional devices.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Computer pin</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Pincer</td>
			<td>DELI Group Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>502 super glue</td>
			<td>DELI Group Co., Ltd.</td>
			<td>7144</td>
			<td></td>
		</tr>
		<tr>
			<td>Drying oven</td>
			<td>Boxun</td>
			<td>GZX-9140MBE</td>
			<td></td>
		</tr>
		<tr>
			<td>Isofluorane</td>
			<td>RWD Life Science</td>
			<td>902-0000-522</td>
			<td></td>
		</tr>
		<tr>
			<td>Stereotaxic apparatus</td>
			<td>RWD Life Science</td>
			<td>900-0068-507</td>
			<td></td>
		</tr>
		<tr>
			<td>Anesthesia apparatus</td>
			<td>RWD Life Science</td>
			<td>902-0000-510</td>
			<td></td>
		</tr>
		<tr>
			<td>Homeothermic Heating Device</td>
			<td>Harvard Apparatus</td>
			<td>K 024509</td>
			<td></td>
		</tr>
		<tr>
			<td>Amplifier Model 3000&#160;</td>
			<td>A-M Systems</td>
			<td>61558</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro1401 Analog Digital converter</td>
			<td>Cambridge Electronic Design Ltd.</td>
			<td>4383</td>
			<td>Data acquisition unit</td>
		</tr>
		<tr>
			<td>Spike2</td>
			<td>Cambridge Electronic Design Ltd.</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

recording eeg in freely moving neonatal rats using a novel method

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such as epilepsy and neurodegenerative disorders. However, it is technically difficult to obtain EEG recordings in neonates as it requires specialized handling and great care. Here, we present a novel method to record EEG in neonatal rat pups (P8-P15). We designed a simple and reliable electrode using computer pin loci; it can be easily implanted into the skull of a rat pup to record high-quality EEG signals in the normal and epileptic brain. Pups were given an intraperitoneal (i.p.) injection of the neurotoxin kainic acid (KA) to induce epileptic seizures. The surgical implantation performed in this procedure is less expensive than other EEG procedures for neonates. This method allows one to record high-quality and stable EEG signals for more than 1 week. Furthermore, this procedure can also be applied to adult rats and mice to study epilepsy or other neurological disorders.

If the above surgical procedures are conducted properly, one channel rat neonatal pup EEG recording will be successfully performed. 10 min after the KA injection, a regular pattern of behavioral signs emerged in the form of irregular movements and scratching, tremors, and loss of balance. Figure 2 shows the representative raw EEG traces and interictal, ictal-tonic, and ictal-clonic expanded traces. Recurrent interictal and ictal EEG discharge patterns started 15-60 min (30 &#177;5.2 min; ...

Watch this Scientific Journal Video about Recording EEG in Freely Moving Neonatal Rats Using a Novel Method at JoVE.com

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

Here, we introduce a novel technique designed to record electroencephalography (EEG) in freely moving neonatal epileptic pups and describe its procedures, features, and applications. This method allows one to record EEG for more than 1 week.

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such ...

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