A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
16.8K Views
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10:07 min
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May 17th, 2017
DOI :
May 17th, 2017
•1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College
Chapters in this video
0:05
Title
1:52
Human Articular Cartilage Tissue Collection and Chondrocyte Culture
3:33
Genomic DNA Extraction
5:47
Dot Blot nalysis
7:55
Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
8:45
Conclusion
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