JoVE Logo
Faculty Resource Center

Sign In

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

DOI :

10.3791/55575-v

April 27th, 2017

April 27th, 2017

10,683 Views

1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre

Proteins often contain multiple domains that can exert different cellular functions. Gene knock-outs (KO) do not consider this functional diversity. Here, we report a recombination-mediated cassette exchange (RMCE)-based structure-function approach in KO embryonic stem cells that allows for the molecular dissection of various functional domains or variants of a protein.

Tags

Structure function Studies

-- Views

Related Videos

article

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

article

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

article

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

article

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

article

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

article

Single-cell Photoconversion in Living Intact Zebrafish

article

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

article

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

article

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

article

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved