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Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

DOI :

10.3791/51764-v

August 23rd, 2014

August 23rd, 2014

10,679 Views

1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

Reporter cell lines offer a means to visualize, track and isolate cells of interest from heterogeneous populations. However, gene-targeting using conventional homologous recombination in human embryonic stem cells is extremely inefficient. Herein, we describe targeting CNS midbrain specific transcription factor PITX3 locus with EGFP using zinc-finger nuclease enhanced homologous recombination.

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Zinc finger Nuclease

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