This work was supported in part by the Van Andel Research Institute and a research grant from the National Institutes of Health (CA181343) to S.B.R.

1. Installing and Running ArrayNinja
<ol>
	<li>Download and install Oracle Virtual Box from www.virtualbox.org.</li>
	<li>Download and uncompress the ArrayNinja virtual machine (VM) from http://research.vai.org/Tools/arrayninja.</li>
	<li>Open Virtual Box and add the ArrayNinja VM by clicking &#39;Machine,&#39; &#39;Add,&#39; and select arrayninja.vbox from the folder where ArrayNinja VM was saved.</li>
	<li>Start ArrayNinja by selecting it inside Virtual Box and clicking the green &#39;start&#39; arrow.</li>
	<li>Virtual Box will open a new window and display a message that the VM can be accessed by navigating the web browser to localhost:2080.&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160; NOTE: A containerized version of ArrayNinja is also available via hub.docker.com/r/bradley.dickson/arrayninja/</li>
</ol>
2. Designing the Array Slide and Source Plate Layout
<ol>
	<li>Under the &#39;Plan a slide layout&#39; heading on the ArrayNinja interface, click on the link that corresponds to the microarray printer being used. 
	NOTE: ArrayNinja has been programmed to mimic the robotic movement of two commonly used microarray printers (see Table 1). Compatibility with the other arrayers can be configured upon request.</li>
	<li>Click inside the &#39;Load plate&#39; dialog box, type &#34;empty&#34;, and click &#39;enter.&#39; See Figure 2 for a screenshot of the ArrayNinja design module.</li>
	<li>Adjust the spot diameter to 275 &#181;m and spot spacing to 375 &#181;m. Adjust the remaining settings (Plate Blocks/plate row, Total plate Rows, replicates, features in y, Super Arrays, SuperA fudge; see Figure 2) to customize how the features will appear on the microarray slide. 
	NOTE: The spot diameter is determined by the size of the microarray pin. As these settings are adjusted, the cartoon slide will update in real-time. Use this cartoon to preview how each setting is modifying the final slide layout.</li>
	<li>After the layout of features on the slide is finalized, mouse over each unique feature and enter the feature identifier in the pop-up dialog box. 
	NOTE: Feature identifiers can consist of numbers, letters, or combinations. This is only required for unique features, and a dialog box will not appear when replicates are selected.</li>
	<li>After all unique features have been assigned an identifier, click &#39;populate.&#39; Enter a name for the slide layout and click &#39;print your plate&#39; to save. A new page will open displaying the number of 384-well source plates needed to fabricate the chosen slide design and a table mapping the physical location of each feature to be loaded in the source plate(s). 
	NOTE: Remember this name, as it will be used to recall this design when analyzing microarray data (see section 6.2). Clicking &#39;print your plate&#39; saves the layout within ArrayNinja.</li>
</ol>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/55912/55912fig2.jpg" /> 
Figure 2: ArrayNinja Design Module. A screen shot of the ArrayNinja design module is shown in the dotted line. The control panel (top) shows all of the parameters that can be altered on the microarray printer. As these parameters are adjusted, the cartoon image of the slide layout (bottom left) updates in real time. After the layout is set, the user can mouse over individual spots to enter unique feature identifiers. ArrayNinja constructs from this user input a map of the position of each feature in the source plate(s) (bottom right) needed to fabricate a specified microarray slide layout. <a href="http://cloudfront.jove.com/files/ftp_upload/55912/55912fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
3. Fabricating Microarrays
<ol>
	<li>Preparing the Source Plate
	<ol>
		<li>Use the map generated with ArrayNinja in section 2.5 to create the 384-well source plate(s). 
		NOTE: Detailed descriptions of peptides queried on this platform can be found elsewhere17.</li>
		<li>Deposit 1 - 2 &#181;L of each feature (e.g., biotinylated histone peptide) into the correct well of the 384-well source plate(s). 
		NOTE: Biotinylated histone peptides are typically deposited from 200 - 400 &#181;M stock solutions, which equates to 10 to 25-fold molar excess of peptide to streptavidin binding sites in a single array spot. This is calculated using the equation: 
		<img alt="Equation" src="/files/ftp_upload/55912/55912eq1.jpg" /> 
		where V is the volume delivered by a pin, [P] is the concentration of the feature being printed, NA is Avogadro&#39;s number, and S is the surface area of a spot. C is the coverage of the slide, expressed as the number of streptavidin molecules per unit area multiplied by three (the average number of available streptavidin binding sites). V and C are obtained by the respective manufacturer&#39;s. Other features may require different concentrations depending on the size of the biomolecule where crowding may be a concern. A range of printing concentrations for each new type of feature should be tested empirically to determine the optimum printing concentration.</li>
		<li>Dilute each feature 10-fold with 1x printing buffer supplemented with 1% bovine serum albumin (BSA). Spin the source plate(s) at 500 x g for 2 min at room temperature. 
		NOTE: The inclusion of fluorescein-labeled biotin (5 &#181;g/&#181;L) in the printing buffer is recommended as a spotting control and as a visual aid to facilitate proper array alignment during analysis (see Figure 4).</li>
	</ol>
	</li>
	<li>Printing Protocol (Figure 3A &#8211; B).
	<ol>
		<li>Prepare the arrayer by emptying the waste collection container and filling the wash solution container and humidifier container with sterile distilled water.</li>
		<li>Enter the parameters used to design the slide in ArrayNinja (section 2 and Figure 2) into the microarray printer control program.</li>
		<li>Using the microarray printer control program, set the washing procedure for a 1 s wash with one immersion. Set the post-wash settings to re-dip the pins 5 times following each wash. Set the humidity to 60%. 
		NOTE: The optimal wash configuration may vary depending on the microarray printer being used. The optimal post-wash pin dip configuration may vary depending on the microarray printer being used.</li>
		<li>Insert functionalized slides (e.g., streptavidin-coated glass) into the substrate platens and place all platens into the platen elevator. Insert the source plate(s) into the plate holder(s) and place into the source plate elevator.</li>
		<li>Click &#39;print&#39;. Monitor the printing process for a few rounds of feature deposition to ensure all wash and dip settings are correct. When the print run is complete, remove the substrate platens from the arrayer. 
		NOTE: When large print runs prohibit completion of blocking steps within one day, printed slides can be incubated in a humidified chamber at 4 &#176;C overnight. Incubate the slides next to a small beaker of water inside a cardboard box sealed with plastic wrap.</li>
		<li>Block the slides with blocking buffer for 30 min at room temperature with mixing.</li>
		<li>Wash the slides 2 x 10 min at room temperature in phosphate buffered saline (PBS), pH 7.6 with mixing. Dry the slides by spinning in a microarray slide centrifuge for 30 s at room temperature. 
		NOTE: For processing a large number of slides at once, a high-throughput microscope slide washing chamber can be used, allowing 50 slides to be washed in parallel.</li>
		<li>For slides designed to be partitioned with wax, proceed to section 4.1. For all other designs, store slides at 4 &#176;C protected from light and moisture. 
		NOTE: Printed biotinylated histone peptides are stable for at least 6 months when stored this way.</li>
	</ol>
	</li>
</ol>
4. Partitioning Microarray Slides
<ol>
	<li>Hydrophobic Wax Pen (Figure 3C)
	<ol>
		<li>Apply wax around the areas that contain features using a wax pen. Allow wax to air dry for 5 min before proceeding to section 5. 
		NOTE: After blocking, the array spots can be very difficult to visualize by eye. The slide design from ArrayNinja can be printed to scale and used as a guide for applying wax.</li>
	</ol>
	</li>
	<li>Silicon gasket (Figure 3D)
	<ol>
		<li>Peel the clear film off the back of the array gasket and place adhesive side down onto the microarray slide.</li>
		<li>Hold the gasket in place for 5 s prior to proceeding to section 5.</li>
	</ol>
	</li>
	<li>Wax Imprint (Figure 3E)
	<ol>
		<li>For slides designed to be partitioned by wax, print a test slide on plain glass using 10% BSA. Use this test slide to optimize the wax imprinter guides or array settings to ensure all features will be within the wax-mold chambers.</li>
		<li>Heat the microarray wax imprinter to 85 &#176;C until all the wax is completely melted, approximately 30 min.</li>
		<li>Insert the slide with the printed side facing down and push the slide all the way to the right guide on the microarray imprinter. Pull up the lever to bring the mold into contact with the surface of the slide. Hold for 2 s. 
		NOTE: The hold time can be altered to achieve optimal wax border thickness.</li>
		<li>Quickly remove the slide and visually inspect the wax borders to ensure all wells are enclosed and that the borders are not so thick that they encroach on the spots. Store slides at 4 &#176;C protected from moisture and light. 
		NOTE: The hold time can be increased or decreased to obtain thicker or thinner borders.</li>
	</ol>
	</li>
</ol>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/55912/55912fig3.jpg" /> 
Figure 3: Microarray Fabrication. (A) Histone peptide microarray fabrication on streptavidin-coated microscope slides using a contact microarray printer. (B) Microarrays fabricated with 3 subarrays of a 48 x 48 grid of peptide features. Separation of (C) 3 subarrays with a hydrophobic wax pen, (D) 2 subarrays with a silicon adhesive, and (E) 48 subarrays with a wax imprint. All microarrays shown are fabricated using 25 x 75 mm microscope slides. <a href="http://cloudfront.jove.com/files/ftp_upload/55912/55912fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
5. Hybridizing a Histone PTM Antibody with a Peptide Microarray
<ol>
	<li>Prepare hybridization buffer (PBS, pH 7.6, 5% BSA, 0.1% Tween-20).</li>
	<li>Equilibrate the slide in hybridization buffer using a hybridization vessel. Completely cover the entire slide in hybridization buffer and incubate for 30 min at 4 &#176;C on an orbital shaker at low speed.</li>
	<li>Prepare a solution containing diluted histone PTM antibody in hybridization buffer. 
	NOTE: In the example data, both histone Antibody #1 and Antibody #2 were diluted 1:1,000 in hybridization buffer. A dilution range similar to that used for immunoblotting is recommended as a starting point.</li>
	<li>Incubate the array with the antibody solution for 1 h at 4 &#176;C. Remove the antibody solution and wash the array 3 times for 5 min at 4 &#176;C with cold PBS, pH 7.6.</li>
	<li>Prepare a 1:5,000 - 1:10,000 dilution of fluorescent dye-conjugated secondary antibody in hybridization buffer.</li>
	<li>Incubate the array with secondary antibody solution for 30 min at 4 &#176;C protected from light. Remove the secondary antibody solution and wash the microarray slide 3 times for 5 min at 4 &#176;C with PBS, pH 7.6. Dip the microarray in a 50-mL conical tube containing 0.1x PBS, pH 7.6 to remove excess salt at room temperature. Dry the slide in a microarray slide centrifuge at room temperature.</li>
	<li>Scan the slide with a microarray scanner at 25 &#181;m resolution or higher, following the microarray scanner manufacturer&#39;s recommended scanning protocol. 
	NOTE: If fluorescein-labeled biotin tracer is present, scan both the green channel (ex: 488 nm, em: 509 nm) and the channel that corresponds to the fluorescent dye-conjugated secondary antibody, typically red (ex: 635 nm, em: 677 nm). The goal of scanning is to obtain single channel .tif files that can be merged into a single .png file (as described in section 6.1).</li>
</ol>
6. Analysis of Microarray Data using ArrayNinja
<ol>
	<li>Preparing a merged microarray image 
	NOTE: The goal of this section is to create a .png image file that merges the two single-channel .tif files (obtained in section 5). This is the only image format compatible with the ArrayNinja analysis module. The following instructions represent one possible way to obtain a merged image file. However, other solutions are available (e.g., the freeware ImageJ).
	<ol>
		<li>From the command line in a bash terminal of a computer with the freeware ImageMagick installed, navigate to the folder that contains the single channel .tif files and copy/paste the following steps, hitting &#39;enter&#39; between each step (6.1.4 - 6.1.7). 
		NOTE: File names in capital letters (e.g., RED_CHANNEL) should be replaced with the file name of the microarray slide images.</li>
		<li>If necessary, first invert the images using the command &#39;convert INPUT.tif -negate OUTPUT.tif&#39;. This is required if the scanner saves .tif files with the signal in white and background in black.
		<ol>
			<li>convert -depth 16 RED_CHANNEL.TIF -clone 0 -channel GB -evaluate set 0 -delete 0 out.png 2&#62;error.file.</li>
			<li>convert - depth 16 CONTROL_CHANNEL.TIF -clone 0 -channel RB -evaluate set 0 -delete 0 outa.png 2&#62;error.file.</li>
			<li>convert -depth 16 CONTROL_CHANNEL.TIF -clone 0 -channel RG -evaluate set 0 -delete 0 outB.png 2&#62;error.file.</li>
			<li>convert out.png outa.png outB.png -set colorspace RGV -combine merged.png. 
			NOTE: A file named &#39;merged.png&#39; will be saved in the same folder as the original .tif files.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Quantifying data using ArrayNinja
	<ol>
		<li>Open ArrayNinja and click on the appropriate microarray printer link under &#34;To quantify images which have a known source plate.&#34; Type the name of the saved slide design (step 2.5) in the &#34;load plate&#34; dialog box and click &#39;enter.&#39; See Figure 4 for a screenshot of the ArrayNinja analysis module.</li>
		<li>Click &#39;Choose File&#39; and navigate to and select the &#39;merged.png&#39; file created in section 6.1. The merged image will load as well as a grid for the slide layout.</li>
		<li>Use the sliders at the bottom of the ArrayNinja control panel to adjust the contrast, brightness, and midpoint. 
		NOTE: These adjustments are for visualization purposes only and have no impact on quantification.</li>
		<li>Select &#34;resolution&#34; and enter the value that matches the resolution of the scanned image. Use the &#34;margin side&#34; and &#34;margin top&#34; to move the grid into alignment with the spots on the array. Adjust as needed to get the grid as closely aligned over each spot as possible.</li>
		<li>Click &#39;Spot Seek&#39; and wait until the button returns to the original grey color. 
		NOTE: This can be repeated several times to center the grid circles on individual spots. The Seek function relaxes the grid toward each spot to fine-tune the alignment.</li>
		<li>Change the &#34;Super Arrays&#34; value to &#34;1&#34; to work up each sub array panel individually (if desired). If using a 4 x 12 wax imprint slide layout (Figure 3E), use the &#34;iPin&#34; &#34;jPin&#34; and &#34;subA&#34; controls to turn off features to analyze any desired combination of 4 x 12 wells. For example, to analyze the top left and top right wells as replicates of each other, enter &#34;1 4&#34; into iPin, &#34;1 1&#34; into jPin and &#34;1 4&#34; into subA. Press &#39;enter&#39;.</li>
		<li>Hover the mouse over individual spots to view the identity of that feature.</li>
		<li>Click &#39;Toggle zoom&#39; to review features more carefully. Select background reference spots by pressing the &#39;R&#39; key while the mouse is over a spot. Reference spots will be highlighted in orange. 
		NOTE: In &#34;toggle zoom&#34; mode, a magnified image of the feature that the mouse is over is displayed in the upper right hand corner. A detailed discussion of additional background correction features in ArrayNinja are discussed elsewhere28.</li>
		<li>Toggle off spots (with widely varied spot morphology or debris that would affect accurate quantification) by pressing the &#39;A&#39; key while hovering the mouse over the affected spots. Inactivated spots will turn white.</li>
		<li>Click &#39;populate&#39; followed by &#39;Submit&#39; to quantify the spots. 
		NOTE: A new tab will open and display a bar graph of data normalized to the brightest spot average. A table below the bar graph contains both the normalized and raw data values. This table can be copied into a spreadsheet for archiving and further analysis.</li>
	</ol>
	</li>
</ol>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/55912/55912fig4.jpg" /> 
Figure 4: ArrayNinja Analysis Module. A screen shot of the ArrayNinja analysis module is shown. The control panel (top left) shows all of the parameters that can be adjusted to visualize the array, find spots, and align a grid over the array image. Hovering the mouse over a feature shows a zoomed-in view (top right) and displays a popup that contains the identification information associated with that feature (bottom). Reference spots selected for background correction are orange. Features to be excluded from downstream analysis are white. ArrayNinja contains a text-based search feature that highlights matching features in yellow, as shown in the example for H4K16. <a href="http://cloudfront.jove.com/files/ftp_upload/55912/55912fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

The authors have nothing to disclose.

Antibody reliability in biomedical research applications is paramount46,47. This is especially true in chromatin biochemistry given the position of antibodies as key tools for the majority of techniques developed to characterize the abundance and distribution of histone PTMs. The protocol presented here details an optimized pipeline for the design, fabrication, and use of peptide microarrays to analyze histone PTM antibody specificity. This pipeline has been used...

Genomic DNA is elegantly packaged inside the eukaryotic cell nucleus with histone proteins to form chromatin. The repeating subunit of chromatin is the nucleosome, which consists of 147 base pairs of DNA wrapped around an octameric core of histone proteins &#8211; H2A, H2B, H3, and H41. Chromatin is broadly organized into loosely packed euchromatin and tightly packed heterochromatin domains. The degree of chromatin compaction regulates the extent to which protein machineries can access the underlying DNA to carry out fundamental DNA-templated processes like replication, transcription, and repair.
Key regulators of genome accessibility in the context of chromatin are PTMs on the unstructured tail and core domains of histone proteins2,3. Histone PTMs function directly by influencing the structure of chromatin4 and indirectly through the recruitment of reader proteins and their associated macromolecular complexes that have chromatin remodeling, enzymatic, and scaffolding activities5. Studies of histone PTM function over the past two decades overwhelmingly suggest these marks play key roles in regulating cell fate, organismal development, and disease initiation/progression. Fueled by advances in mass spectrometry-based proteomic technology, more than 20 unique histone PTMs on more than 80 distinct histone residues have been discovered6. Notably, these modifications often occur in combinations, and consistent with the &#34;histone code&#34; hypothesis, numerous studies suggest that reader proteins are targeted to discrete regions of chromatin through recognition of specific combinations of histone PTMs7,8,9. A key challenge moving forward will be to assign functions to the growing list of histone PTMs and to determine how specific combinations of histone PTMs orchestrate the dynamic functions associated with chromatin.
Antibodies are the lynchpin reagents for the detection of histone PTMs. As such, more than 1,000 histone PTM-specific antibodies have been commercially developed for use in chromatin biochemistry research. With the rapid development of high-throughput DNA sequencing technology, these reagents are being used extensively by individual investigators and large-scale epigenomics &#34;roadmap&#34; initiatives (e.g., ENCODE and BLUEPRINT) in ChIP-seq (chromatin immunoprecipitation coupled with next-generation sequencing) pipelines to generate high-resolution spatial maps of histone PTM distribution genome-wide10,11. However, recent studies have shown that the specificity of histone PTM antibodies can be highly variable and that these reagents exhibit unfavorable properties such as off-target epitope recognition, strong positive and negative influence by neighboring PTMs, and difficulty discriminating the modification order on a particular residue (e.g., mono-, di-, or tri-methyllysine)12,13,14,15,16,17,18. Therefore, rigorous quality control of histone PTM-specific antibody reagents is necessary to accurately interpret data generated with these valuable reagents.
Microarray technology enables the simultaneous interrogation of thousands of macromolecular interactions in a high-throughput, reproducible, and miniaturized format. For this reason, a variety of microarray platforms have been created to analyze protein-DNA19,20, protein-protein 21, and protein-peptide interactions22. Indeed, histone peptide microarrays have emerged as an informative discovery platform for chromatin biochemistry research, enabling high-throughput profiling of the writers, erasers, and readers of histone PTMs15,23,24, and also for the analysis of histone antibody specificity17,25. Beyond their application in chromatin and epigenetics research, histone peptide arrays have potential utility as a diagnostic/prognostic test for systemic lupus erythematosus and other autoimmune diseases where anti-chromatin autoantibodies are generated26,27.
Here, we describe an integrated pipeline that we have developed for designing, fabricating, and querying histone peptide microarrays to generate specificity profiles for antibodies that recognize histones and their PTMs. The pipeline is facilitated by ArrayNinja, an open-source, interactive software application that we recently developed, which integrates the design and analysis stages of microarray experiments28. ArrayNinja works best in Google Chrome. Briefly, a robotic contact microarray printer is used to deposit a library of biotin-conjugated histone peptides at defined positions on streptavidin-coated glass microscope slides. Arrays can then be used in a competitive and parallel assay format to interrogate antibody-epitope interactions (Figure 1). The peptide library consists of hundreds of unique synthetic peptides harboring PTMs (lysine acetylation, lysine/arginine methylation, and serine/threonine phosphorylation) alone and in relevant combinations largely derived from proteomics datasets. Methods for peptide synthesis and validation are detailed elsewhere23. Data generated from our ongoing histone PTM antibody screening efforts utilizing this array platform are archived on a public web resource, the Histone Antibody Specificity Database (www.histoneantibodies.com). Notably, histone peptide microarrays fabricated with variations of this protocol have also been used extensively to characterize the activity of histone PTM reader domains8,29,30,31,32,33,34,35,36,37 and more recently to profile histone PTM writer and eraser activities24.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/55912/55912fig1.jpg" /> 
Figure 1: Cartoon Depiction of the Stepwise Procedure for Antibody Screening on a Histone Peptide microarray. Biotinylated histone peptides harboring defined post-translational modifications (red and blue circles) are co-printed with biotin-fluorescein on streptavidin-coated glass. Positive interactions are visualized as red fluorescence. <a href="http://cloudflare.jove.com/files/ftp_upload/55912/55912fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

<table><tbody><tr><td>Printing Buffer</td><td>ArrayIt</td><td>PPB</td><td></td></tr><tr><td>BSA</td><td>Omnipure</td><td>2390</td><td></td></tr><tr><td>Streptavidin-coated glass microscope slides</td><td>Greiner Bio-one</td><td>439003-25</td><td></td></tr><tr><td>polypropylene 384 well plate</td><td>Greiner Bio-one</td><td>784201</td><td></td></tr><tr><td>Biotin-fluorescein</td><td>Sigma</td><td>53608</td><td></td></tr><tr><td>contact microarray printer</td><td>Aushon</td><td>2470</td><td>Aushon 2470 Microarray Printer</td></tr><tr><td>contact microarray printer</td><td>Gene Machines</td><td>OmniGrid 100</td><td>OmniGrid Microarray Printer</td></tr><tr><td>PBS</td><td>Invitrogen</td><td>14190</td><td></td></tr><tr><td>Blocking Buffer</td><td>ArrayIt</td><td>SBB</td><td></td></tr><tr><td>Hydrophobic wax pen</td><td>Vector Labs</td><td>H-4000</td><td>ImmEdge Hydrophobic Barrier PAP Pen</td></tr><tr><td>Silicon Gasket</td><td>Grace Bio-labs</td><td>622511</td><td></td></tr><tr><td>Hybridization Vessel</td><td>Thermo Scientific</td><td>267061</td><td>or similar vessel</td></tr><tr><td>Fluorescent-dye conjugated secondary antibody</td><td>Life Technologies</td><td>A-21244</td><td>Alexa Fluor 647 (anti-rabbit)</td></tr><tr><td>Fluorescent-dye conjugated secondary antibody</td><td>Life Technologies</td><td>A-21235</td><td>Alexa Fluor 647 (anti-mouse)</td></tr><tr><td>Wax Imprinter</td><td>ArrayIt</td><td>MSI48</td><td></td></tr><tr><td>Tween-20</td><td>Omnipure</td><td>9490</td><td></td></tr><tr><td>Microarray Scanner</td><td>Innopsys</td><td>InnoScan 1100AL</td><td>or equivalent microarray scanner</td></tr><tr><td>EipTitan Histone Peptide Microarray</td><td>Epicypher</td><td>112001</td><td></td></tr><tr><td>AbSurance Pro Histone Peptide Microarray</td><td>Millipore</td><td>16668</td><td></td></tr><tr><td>MODified Histone Peptide Array</td><td>Active Motif</td><td>13001</td><td></td></tr><tr><td>Histone Code Peptide Microarrays</td><td>JPT</td><td>His_MA_01</td><td></td></tr><tr><td>Wax</td><td>Royal Oak</td><td>GulfWax</td><td>for wax imprinter</td></tr><tr><td>Humidified Microarray Slide Hybridization Chamber</td><td>VWR</td><td>97000-284</td><td></td></tr><tr><td>High throughput microscope slide washing chamber</td><td>ArrayIt</td><td>HTW</td><td></td></tr><tr><td>Microscope slide centrifuge</td><td>VWR</td><td>93000-204</td><td></td></tr><tr><td>Antibody 1</td><td>Abcam</td><td>8898</td><td></td></tr><tr><td>Antibody 2</td><td>Millipore</td><td>07-473</td><td></td></tr><tr><td>Biotinylated histone peptide</td><td>EpiCypher</td><td>12-0001</td><td>Example peptide. Similar peptides with various modifications are available from several commercial sources.</td></tr><tr><td>ImageMagick</td><td></td><td></td><td>https://www.imagemagick.org/script/index.php</td></tr><tr><td>ArrayNinja</td><td></td><td></td><td>https://rothbartlab.vai.org/tools/</td></tr></tbody></table>

analysis of histone antibody specificity with peptide microarrays

Post-translational modifications (PTMs) on histone proteins are widely studied for their roles in regulating chromatin structure and gene expression. The mass production and distribution of antibodies specific to histone PTMs has greatly facilitated research on these marks. As histone PTM antibodies are key reagents for many chromatin biochemistry applications, rigorous analysis of antibody specificity is necessary for accurate data interpretation and continued progress in the field. This protocol describes an integrated pipeline for the design, fabrication and use of peptide microarrays for profiling the specificity of histone antibodies. The design and analysis aspects of this procedure are facilitated by ArrayNinja, an open-source and interactive software package we recently developed to streamline the customization of microarray print formats. This pipeline has been used to screen a large number of commercially available and widely used histone PTM antibodies, and data generated from these experiments are freely available through an online and expanding Histone Antibody Specificity Database. Beyond histones, the general methodology described herein can be applied broadly to the analysis of PTM-specific antibodies.

This protocol has been used to design and fabricate a peptide microarray platform for the analysis of histone PTM antibody specificity. The array queries a library of more than 300 unique peptide features (20 - 40 residues in length) representing many of the known combinations of PTMs found on core and variant histone proteins38. This pipeline has been a workhorse for the screening of many widely used and commercially available histone PTM antibodies, and full data...

Watch this Scientific Journal Video about Analysis of Histone Antibody Specificity with Peptide Microarrays at JoVE.com

Analysis of Histone Antibody Specificity with Peptide Microarrays

This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications.

Post-translational modifications (PTMs) on histone proteins are widely studied for their roles in regulating chromatin structure and gene expression. The ...

analysis-of-histone-antibody-specificity-with-peptide-microarrays

Research

JoVE Journal

Biochemistry

40.0K Views.  Van Andel Research Institute. This manuscript describes methods for applying peptide microarray technology to specificity profiling of antibodies that recognize histones and their post-translational modifications. 

Video: Analysis of Histone Antibody Specificity with Peptide Microarrays

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

In organ transplantation, the function and longevity of the graft critically rely on the success of controlling immunological rejection reactivity against human leukocyte antigens (HLA). Histocompatibility guidelines are based on laboratory tests of anti-HLA immunity, which presents either as pre-existing or de novo generated HLA antibodies that constitute a major transplantation barrier. Current tests are built on a single-antigen beads (SAB) platform using a fixed set of ~100 preselected recombinant HLA antigens to probe transplant sera. However, in humans there exist a far greater variety of HLA types, with no two individuals other than identical twins who can share the same combination of HLA sequences. While advanced technologies for HLA typing and direct sequencing can precisely capture any mismatches in DNA sequence between a donor's and recipient's HLA, the SAB assay, due to its limited variety in sequence representation, is unable to precisely detect alloantibodies specifically against the donor HLA mismatches. We sought to develop a complementary method using a different technology to detect and characterize anti-donor HLA antibodies on a personalized basis. The screening tool is a custom peptide array of donor HLA-derived sequences for probing post-transplant sera of the organ recipient to assess the risk for antibody-mediated rejection. On a single array for one donor-recipient pair, up to 600 unique peptides are made based on the donor's HLA protein sequences, each peptide carrying at least one mismatched residue in a 15-amino acid sequence. In our pilot experiments to compare antigen patterns for pre- and post-transplant sera on these arrays, we were able to detect anti-HLA signals with the resolution that also allowed us to pinpoint the immune epitopes involved. These personalized antigen arrays allow high-resolution detection of donor-specific HLA epitopes in organ transplantation.

Mismatches in human leukocyte antigen (HLA) sequences between organ donor and recipient pairs are the major cause of antibody-mediated rejection in organ transplantation. Here we present the use of custom antigen arrays that are based on individual donors' HLA sequences to probe anti-donor HLA alloantibodies in organ recipients.

In organ transplantation, the function and longevity of the graft critically rely on the success of controlling immunological rejection reactivity against ...

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics is an emerging field that fuels and guides the development of vaccines and immunotherapies. More specifically, it refers to the science of investigating the composition of peptides presented by major histocompatibility complex (MHC) class I and class II molecules using mass spectrometry (MS) technology platforms. Among all the steps in an MS-based immunopeptidomics workflow, sample preparation is critically important for capturing high-quality data of therapeutic relevance. Here, step-by-step instructions are described to isolate MHC class I and II-associated peptides by immunoaffinity purification from quality control samples, from mouse (EL4 and A20), and human (JY) cell lines more specifically. The various reagents and specific antibodies are thoroughly described to isolate MHC-associated peptides from these cell lines, including the steps to verify the beads-binding efficiency of the antibody and the elution efficiency of the MHC-peptide complexes from the beads. The protocol can be used to establish and standardize an immunopeptidomics workflow, as well as to benchmark new protocols. Moreover, the protocol represents a great starting point for any non-experts in addition to foster the intra- and inter-laboratory reproducibility of the sample preparation procedure in immunopeptidomics.

Here, we present a protocol for the purification of MHC class I and class II peptide complexes from mouse and human cell lines providing high-quality immunopeptidomics data. The protocol focuses on sample preparation using commercially available antibodies.

Immunopeptidomics is an emerging field that fuels and guides the development of vaccines and immunotherapies. More specifically, it refers to the science ...

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

The identification of an antigenic epitope by the immune system allows for the understanding of the protective mechanism of neutralizing antibodies that may facilitate the development of vaccines and peptide drugs. Peptide scanning is a simple and efficient method that straightforwardly maps the linear epitope recognized by a monoclonal antibody (mAb). Here, the authors present an epitope determination methodology involving serially truncated recombinant proteins, synthetic peptide design, and dot-blot hybridization for the antigenic recognition of nervous necrosis virus coat protein using a neutralizing mAb. This technique relies on the dot-blot hybridization of synthetic peptides and mAbs on a polyvinylidene fluoride (PVDF) membrane. The minimum antigenic region of a viral coat protein recognized by the RG-M56 mAb can be narrowed down by step-by-step trimmed peptide mapping onto a 6-mer peptide epitope. In addition, alanine scanning mutagenesis and residue substitution can be performed to characterize the binding significance of each amino acid residue making up the epitope. The residues flanking the epitope site were found to play critical roles in peptide conformation regulation. The identified epitope peptide may be used to form crystals of epitope peptide-antibody complexes for an x-ray diffraction study and functional competition, or for therapeutics.

Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.

The identification of an antigenic epitope by the immune system allows for the understanding of the protective mechanism of neutralizing antibodies that ...

Immunology and Infection

Zika Virus Specific Diagnostic Epitope Discovery

High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for drug discovery, therapeutic target identification, and developing of diagnostics. Here, we present a protocol to discover specific Zika virus (ZIKV) diagnostic peptides using a high-density peptide microarray. A human serum sample validated for ZIKV infection was incubated with a high-density peptide microarray containing the entire ZIKV protein translated into 3,423 unique 15 linear amino acid (aa) residues with a 14-aa residue overlap printed in duplicate. Staining with different secondary antibodies within the same array, we detected peptides that bind to Immunoglobulin M (IgM) and Immunoglobulin G (IgG) antibodies present in serum. These peptides were selected for further validation experiments. In this protocol, we describe the strategy followed to design, process, and analyze a high-density peptide microarray.

In this protocol, we describe a technique to discover Zika virus specific diagnostic peptides using a high-density peptide microarray. This protocol can readly be adapted for other emerging infectious diseases.

High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for ...

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. Glycosylation of proteins is the most prevalent post-translational modification found on proteins, and leads diversified modifications of the physical, chemical, and biological properties of proteins. Because the glycosylation machinery is particularly susceptible to disease progression and malignant transformation, aberrant glycosylation has been recognized as early detection biomarkers for cancer and other diseases. However, current methods to study protein glycosylation typically are too complicated or expensive for use in most normal laboratory or clinical settings and a more practical method to study protein glycosylation is needed. The new protocol described in this study makes use of a chemically blocked antibody microarray with glycan-binding protein (GBP) detection and significantly reduces the time, cost, and lab equipment requirements needed to study protein glycosylation. In this method, multiple immobilized glycoprotein-specific antibodies are printed directly onto the microarray slides and the N-glycans on the antibodies are blocked. The blocked, immobilized glycoprotein-specific antibodies are able to capture and isolate glycoproteins from a complex sample that is applied directly onto the microarray slides. Glycan detection then can be performed by the application of biotinylated lectins and other GBPs to the microarray slide, while binding levels can be determined using Dylight 549-Streptavidin. Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed.
Introduction
Glycosylation of protein, which is the most ubiquitous post-translational modification on proteins, modifies the physical, chemical, and biological properties of a protein, and plays a fundamental role in various biological processes1-6. Because the glycosylation machinery is particularly susceptible to disease progression and malignant transformation, aberrant glycosylation has been recognized as early detection biomarkers for cancer and other diseases 7-12. In fact, most current cancer biomarkers, such as the L3 fraction of &alpha;-1 fetoprotein (AFP) for hepatocellular carcinoma 13-15, and CA199 for pancreatic cancer 16, 17 are all aberrant glycan moieties on glycoproteins. However, methods to study protein glycosylation have been complicated, and not suitable for routine laboratory and clinical settings. Chen et al. has recently invented a chemically blocked antibody microarray with a glycan-binding protein (GBP) detection method for high-throughput and multiplexed profile glycosylation of native glycoproteins in a complex sample 18. In this affinity based microarray method, multiple immobilized glycoprotein-specific antibodies capture and isolate glycoproteins from the complex mixture directly on the microarray slide, and the glycans on each individual captured protein are measured by GBPs. Because all normal antibodies contain N-glycans which could be recognized by most GBPs, the critical step of this method is to chemically block the glycans on the antibodies from binding to GBP. In the procedure, the cis-diol groups of the glycans on the antibodies were first oxidized to aldehyde groups by using NaIO4 in sodium acetate buffer avoiding light. The aldehyde groups were then conjugated to the hydrazide group of a cross-linker, 4-(4-N-MaleimidoPhenyl)butyric acid Hydrazide HCl (MPBH), followed by the conjugation of a dipeptide, Cys-Gly, to the maleimide group of the MPBH. Thus, the cis-diol groups on glycans of antibodies were converted into bulky none hydroxyl groups, which hindered the lectins and other GBPs bindings to the capture antibodies. This blocking procedure makes the GBPs and lectins bind only to the glycans of captured proteins. After this chemically blocking, serum samples were incubated with the antibody microarray, followed by the glycans detection by using different biotinylated lectins and GBPs, and visualized with Cy3-streptavidin. The parallel use of an antibody panel and multiple lectin probing provides discrete glycosylation profiles of multiple proteins in a given sample 18-20. This method has been used successfully in multiple different labs 1, 7, 13, 19-31. However, stability of MPBH and Cys-Gly, complicated and extended procedure in this method affect the reproducibility, effectiveness and efficiency of the method. In this new protocol, we replaced both MPBH and Cys-Gly with one much more stable reagent glutamic acid hydrazide (Glu-hydrazide), which significantly improved the reproducibility of the method, simplified and shorten the whole procedure so that the it can be completed within one working day. In this new protocol, we describe the detailed procedure of the protocol which can be readily adopted by normal labs for routine protein glycosylation study and techniques which are necessary to obtain reproducible and repeatable results.

In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.

 In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that ...

Biology

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Chromatin is a highly dynamic nucleoprotein complex made of DNA and proteins that controls various DNA-dependent processes. Chromatin structure and function at specific regions is regulated by the local enrichment of histone post-translational modifications (hPTMs) and variants, chromatin-binding proteins, including transcription factors, and DNA methylation. The proteomic characterization of chromatin composition at distinct functional regions has been so far hampered by the lack of efficient protocols to enrich such domains at the appropriate purity and amount for the subsequent in-depth analysis by Mass Spectrometry (MS). We describe here a newly designed chromatin proteomics strategy, named ChroP (Chromatin Proteomics), whereby a preparative chromatin immunoprecipitation is used to isolate distinct chromatin regions whose features, in terms of hPTMs, variants and co-associated non-histonic proteins, are analyzed by MS. We illustrate here the setting up of ChroP for the enrichment and analysis of transcriptionally silent heterochromatic regions, marked by the presence of tri-methylation of lysine 9 on histone H3. The results achieved demonstrate the potential of ChroP in thoroughly characterizing the heterochromatin proteome and prove it as a powerful analytical strategy for understanding how the distinct protein determinants of chromatin interact and synergize to establish locus-specific structural and functional configurations.

By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.

Chromatin is a highly dynamic nucleoprotein complex made of DNA and proteins that controls various DNA-dependent processes. Chromatin structure and ...

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design1,2. Here, a peptide-MHC II binding assay is scaled to 384-well format. The scaled down protocol reduces reagent costs by 75% and is higher throughput than previously described 96-well protocols1,3-5. Specifically, the experimental design permits robust and reproducible analysis of up to 15 peptides against one MHC II allele per 384-well ELISA plate. Using a single liquid handling robot, this method allows one researcher to analyze approximately ninety test peptides in triplicate over a range of eight concentrations and four MHC II allele types in less than 48 hr. Others working in the fields of protein deimmunization or vaccine design and development may find the protocol to be useful in facilitating their own work. In particular, the step-by-step instructions and the visual format of JoVE should allow other users to quickly and easily establish this methodology in their own labs.

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design. &#160;Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or ...

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Protein-protein interactions mediate most of the processes in the living cell and control homeostasis of the organism. Impaired protein interactions may result in disease, making protein interactions important drug targets. It is thus highly important to understand these interactions at the molecular level. Protein interactions are studied using a variety of techniques ranging from cellular and biochemical assays to quantitative biophysical assays, and these may be performed either with full-length proteins, with protein domains or with peptides. Peptides serve as excellent tools to study protein interactions since peptides can be easily synthesized and allow the focusing on specific interaction sites. Peptide arrays enable the identification of the interaction sites between two proteins as well as screening for peptides that bind the target protein for therapeutic purposes. They also allow high throughput SAR studies. For identification of binding sites, a typical peptide array usually contains partly overlapping 10-20 residues peptides derived from the full sequences of one or more partner proteins of the desired target protein. Screening the array for binding the target protein reveals the binding peptides, corresponding to the binding sites in the partner proteins, in an easy and fast method using only small amount of protein.
In this article we describe a protocol for screening peptide arrays for mapping the interaction sites between a target protein and its partners. The peptide array is designed based on the sequences of the partner proteins taking into account their secondary structures. The arrays used in this protocol were Celluspots arrays prepared by INTAVIS Bioanalytical Instruments. The array is blocked to prevent unspecific binding and then incubated with the studied protein. Detection using an antibody reveals the binding peptides corresponding to the specific interaction sites between the proteins.

Peptide array screening is a high throughput assay for identifying protein-protein interaction sites. This allows mapping multiple interactions of a target protein and can serve as a method for identifying sites for inhibitors that target a protein. Here we describe a protocol for screening and analyzing peptide arrays.

Protein-protein interactions mediate most of the processes in the living cell and control homeostasis of the organism. Impaired protein interactions may ...

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Lysine methylation is an emerging post-translation modification and it has been identified on several histone and non-histone proteins, where it plays crucial roles in cell development and many diseases. Approximately 5,000 lysine methylation sites were identified on different proteins, which are set by few dozens of protein lysine methyltransferases. This suggests that each PKMT methylates multiple proteins, however till now only one or two substrates have been identified for several of these enzymes. To approach this problem, we have introduced peptide array based substrate specificity analyses of PKMTs. Peptide arrays are powerful tools to characterize the specificity of PKMTs because methylation of several substrates with different sequences can be tested on one array. We synthesized peptide arrays on cellulose membrane using an Intavis SPOT synthesizer and analyzed the specificity of various PKMTs. Based on the results, for several of these enzymes, novel substrates could be identified. For example, for NSD1 by employing peptide arrays, we showed that it methylates K44 of H4 instead of the reported H4K20 and in addition H1.5K168 is the highly preferred substrate over the previously known H3K36. Hence, peptide arrays are powerful tools to biochemically characterize the PKMTs.

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

Lysine methylation is an emerging post-translation modification and it has been identified on several histone and non-histone proteins, where it plays ...

A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients

Source: Liu, P., et al. <a href="https://app.jove.com/v/56278">Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation.</a> J. Vis. Exp. (2017).
This video demonstrates an assay to detect anti-donor HLA alloantibodies in organ recipients. A peptide array is synthesized to represent potential epitopes for antibody-mediated organ rejection. The array is incubated with the recipient&#39;s serum, containing alloantibodies that bind to their target epitopes. Antibody binding is detected using chemiluminescence to identify donor-specific HLA epitopes that incite alloantibody reactivity.

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Design of Custom Array Layout and ...