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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

DOI :

10.3791/51308-v

March 25th, 2014

March 25th, 2014

14,591 Views

1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design.  Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.

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MHC II Binding Assay

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