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Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

DOI :

10.3791/55991-v

October 10th, 2017

October 10th, 2017

12,852 Views

1Department of Microbiology and Molecular Genetics, Michigan State University, 2Department of Physiology, Michigan State University

Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.

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In Vivo Monitoring

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