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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

DOI :

10.3791/3293-v

8:52 min

September 28th, 2011

September 28th, 2011

18,726 Views

1Department of Pediatrics, University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 3Department of Pediatrics and Microbiology, University of Texas Southwestern Medical Center

A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.

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RNA Isolation

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