JoVE Logo
Faculty Resource Center

Sign In

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

DOI :

10.3791/55994-v

August 25th, 2017

August 25th, 2017

11,218 Views

1Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, 2Department of Molecular & Integrative Physiology, University of Kansas Medical Center

Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.

Tags

Intravenous Administration

-- Views

Related Videos

article

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models

article

Methods for Intravenous Self Administration in a Mouse Model

article

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

article

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

article

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

article

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

article

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

article

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

article

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

article

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved