Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

13.2K Views

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08:33 min

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February 28th, 2016

DOI :

10.3791/53670-v

February 28th, 2016

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Anke Van der Perren¹, Cindy Casteels², Koen Van Laere², Rik Gijsbers³^,⁴, Chris Van den Haute¹^,⁴, Veerle Baekelandt¹

¹Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, ²Division of Nuclear Medicine, Leuven University Hospital - KU Leuven, ³Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, ⁴Leuven Viral Vector Core, KU Leuven

Summary

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Keywords Parkinson s Disease

Alpha synuclein

Rat Model

Stereotactic Injection

Recombinant Adeno associated Viral Vector

Viral Vector based Animal Model

Pre clinical Drug Testing

Neurodegenerative Disorders

Brain Region Targeting

High Transgene Expression

Microinjection Syringe

Recombinant AAV

Chapters in this video

0:05

Title

1:11

Stereotactic Injection of rAAV-α-SYN into the Rat Substantia Nigra

5:18

Results: Neurodegeneration Induced by rAAV2/7 α-SYN

7:30

Conclusion

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